FINALLY.

Those of you who saw how dejected and generally cranky I was this weekend have heard the story. This weekend, I was in the lab for both weekend days for a few hours struggling to get a PCR to work. A super basic PCR, which itself is already a super basic lab routine. This was both incredibly frustrating and almost embarrassing. Worse, it wasn't even really my job to handle this particular PCR. It was on a line of mice I'm not supposed to be responsible for and that someone else needed for experiments. But my boss pulled a psycho card and determined we needed to work on this NOW NOW NOW, and there went several hours of weekend. At least I made up for missing work for class?

Anyway, the PCR that would not work has been put to bed, finally, following new-ish primers and a different thermo-cycling protocol. I did a different PCR today that worked perfectly and nearly cried with joy just to remember that this is a tool that usually works. By the time I finally vanquished the troublesome PCR, I was so relieved, I could have just closed down my desk and left, and fuck the consequences 'cause I was DONE.

I'll make a point here to say that I am still at work. But I'm posting to LJ! Such is my reckless defiance!