FINALLY.

[trinityvixen]

Those of you who saw how dejected and generally cranky I was this weekend have heard the story. This weekend, I was in the lab for both weekend days for a few hours struggling to get a PCR to work. A super basic PCR, which itself is already a super basic lab routine. This was both incredibly frustrating and almost embarrassing. Worse, it wasn't even really my job to handle this particular PCR. It was on a line of mice I'm not supposed to be responsible for and that someone else needed for experiments. But my boss pulled a psycho card and determined we needed to work on this NOW NOW NOW, and there went several hours of weekend. At least I made up for missing work for class?

Anyway, the PCR that would not work has been put to bed, finally, following new-ish primers and a different thermo-cycling protocol. I did a different PCR today that worked perfectly and nearly cried with joy just to remember that this is a tool that usually works. By the time I finally vanquished the troublesome PCR, I was so relieved, I could have just closed down my desk and left, and fuck the consequences 'cause I was DONE.

I'll make a point here to say that I am still at work. But I'm posting to LJ! Such is my reckless defiance!

Comments

[ivy03.livejournal.com]

I encourage your reckless defiance!

[xannoside.livejournal.com]

I'm really glad that worked out *finally*!

Your boss better appreciate it.... >

[ecmyers.livejournal.com]

I blame Contagion. It made science not work for a little while.

[negativeq.livejournal.com]

Excellent hypothesis! Now, how will you test this? Make sure you state your independent and dependent variables, controls, constant factors and how you will collect data.

(Can you tell what topic I am covering tomorrow?)